Microbiology: Bacteria and Fresh Yogurt Slide

bacteriuml Morphology Demonica britt Microbiology DL1 March 23, 2013 Abstract This lab was performed to identify and familiarize with a microscope mend precisely spy various bacteriuml shapes and their arrangements in divergent types of specimens of bacterium. The microscope move and capabilities were understandably place and used successfully and the bacteria were clearly illustrated video display the bacterial shapes and arrangements with all the appropriate ebullition being utilized.Through various ebullitions development 10x, 40x and 100x oil entry lenses, the bacteria specimens, along with fresh and brisk yoghourt, demonstrate full optical optical views of their shapes and how the different types were displayed at different levels of magnification. usance The purpose of the essay was to gain full knowledge and experience of run a microscope while being able to successfully visualize different types of bacterial and yoghurt specimens shapes and arrangements us ing several magnification techniques by way of 10x, 40x,100x oil immersion lenses and a light source.The of import purpose was to observe the shapes and arrangements of microbial bacteria and yogurt. Procedure The lab touch on self-provided and labpaq materials to perform several exercises to obtain the purpose of the lab. The lab began with the proper identification of all components of the microscope and their functions. This allowed for preparation of the endive of being able to view specimens at various magnification levels and recognizing their different shapes and how they are arranged contingent upon those identified within the lab itself and the microbiology textbook.Several different slips were discover under 10x and 40x lens magnification Paramecium conjugation, Yeast, Amoeba Proteus, Ascaris eggs, Anabaena, and Penicillium. This allowed vivid illustrations of the specimens notating their shapes and how they are arranged. The bacteria were find through the eyepiece at the appropriate focus, resolution, and contrast for maximum visibility. The adjoining part of the lab exercise was observance under an 100x oil immersion lens for much vigilant slides Bacteria Coccus form, Bacteria spirillum, and Bacteria Bacillus form while still maintaining to observe the shapes and arrangements.Additionally, the fresh yogurt slide that was sitting for 24 hours in a dark, warm localisation principle was obtained for the next part of the lab experiment. The fresh yogurt slide was prepared by using a toothpick to place a small add up onto a fresh, clean slide with a slide cover hardened on top. This was observed for comparison to the prepared yogurt slide include in the lab for any variations in forms. Upon completion of performing the lab, the prepared slides were safely put away, fresh slide washed carefully, fresh yogurt specimen safely discarded, and the microscope cleaned and returned to be stored with the protective cover.Data/Observations (Data Tab les & Photos of Labeled Pics & Observations) The bacteria slides clearly displayed the various types of bacteria shapes and showed how each follow a specified arrangement. infra the lowest magnification the object is relatively smaller and not as easy to hitch the full format. Whereas the higher the magnification, the bigger and more heighten the view of the bacteria becomes making the shapes and arrangements relatively obvious. It appeared to become clearer the bigger the object projected to my eye.It became life size in a sense where as it was an image that could be clearly defined, described and duplicated if necessary. The fresh yogurt slide that was set for 24 hours was a more enhanced feature for observing bacteria in yogurt. Its view was very detailed and its shape more recognizable. While the prepared yogurt slide was a more lite view and the color appearing duller. It was visible to me that bacteria in yogurt was more spherical in shape, cocci. Results A. What are the advantages of using bleach as a disinfectant? The disadvantages? The advantages of using 70% alcohol?The disadvantages? Bleach is a common household disinfectant that kills 99. 9 percent of germs whereas others croupenot approach this effectiveness. It can be used to sanitize. It can be a disadvantage as it can be inactivated by presence of an organic matter and it has a strong odor and it has a short life in the liquidness form that can be sensitive to heat and sunlight. The advantages of using 70% bleach is that it can be capable of killing most bacteria which is safe for skin contact and it prevents dehydration and the alcohol part of it imply the cells in various ways.Some disadvantages are that they are hazardous which contain compounds that are not safe and toxic to human form. B. List three reasons wherefore you might choose to stain a particular slide quite an than view it as a wet mount. C. Define the following term Chromophore Acidic Dye Basic Dye D. What is the diffe rence between put and indirect staining? E. What is heat haunt? F. Why is it necessary to verify that your specimens are completely air dried prior to heat fixing? G.Describe what you observed in your plaque mark wet mount, direct stain slide, and indirectly dye slide. What were the similarities? What were the differences? H. Describe what you observed in your brass instrument smear wet mount, direct stained slide, and indirectly stained slide. What were the similarities? What were the differences? I. Describe what you observed in your yeast wet mount, direct stained slide, and indirectly stained slide. What were the similarities? What were the differences? J. Were the cell types the same in all three specimen sets yeast, laque, and cheek? How were they similar? How were they different? Conclusion/Discussion Upon performing and completing the experiment I learned that the microscope is a very delicate tool that allows the power of viewing specimens too small for the human ey e. With ad fulling the focus, contrast, and resolution, the bacteria become more visible to the eye. On top of that, viewing the specifications at different magnifications the bacteria shapes and arrangements become more present within the specimen.Bacteria comes in different forms and shapes and just by arrangement alone, they can be classified morphologically. It was also visual that there are differences in a fresh slide containing bacteria compared with a slide already prepared. I did not expect to see the differences so vividly displayed, but after using the microscope it was determined that anything not visible to the naked eye still has the capability to be seen and the microscope is the blameless tool to use to be able to do so.